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Cannabis produces more than 100 cannabinoids ( Baron, 2018) that have many effects in the human body, including modulation of mood, memory and the immune response. ruderalis, which are differentiated by key physical characteristics and production of cannabinoids ( Hillig, 2005 Rana, 2010 Atakan, 2012). There are three main subspecies of cannabis: C. Cannabis, commonly referred as marijuana, is a flowering plant belonging to the family Cannabaceae. This study provides an optimized protocol for aqueous CaSE preparation containing biologically active cannabinoids that can be used for in vitro experimentation of cannabis smoke and its potential impact on various indices of pulmonary health.Ĭannabis has been used for medical purposes for thousands of years ( Hillig, 2005 Rana, 2010 Atakan, 2012). Finally, this standardized preparation of CaSE induces an inflammatory response in human lung fibroblasts. Using a functional assay in cells for CB1 receptors, the major target of cannabinoids, we found that this CaSE contains Δ 9-THC which activates CB1 receptors. We also observed by LC-MS/MS that CaSE preparation with methanol contains CBD. First, we determined the concentration of Δ 9-THC, one of the major cannabinoids, by ELISA and found that addition of methanol to the cell culture media during generation of the aqueous smoke extract significantly increased the amount of Δ 9-THC. Therefore, we developed a standardized protocol for the generation of cannabis smoke extract (CaSE) to investigate its effect on cellular mechanisms in vitro. In order to understand the impact of cannabis smoke on pathological mechanisms associated with adverse respiratory outcomes, the development of in vitro surrogates of cannabis smoke exposure is needed. These compounds are hydrophobic and not present in aqueous solutions. Besides the combustion products present in cannabis smoke, cannabis also contains cannabinoids including Δ 9-tetrahydrocannabinol (Δ 9-THC) and cannabidiol (CBD). Although the impact of tobacco smoke on respiratory health is well-known, the consequence of cannabis smoke on the respiratory system and, in particular, the inflammatory response is unclear. Combustion of cannabis smoke generates many of the same chemicals as tobacco smoke. Smoking cannabis in the form of a joint is the most common mode of cannabis consumption. There has been a rapid increase in the number of countries legalizing cannabis for both recreational and medicinal purposes. Cannabis (marijuana) is the most commonly used illicit product in the world and is the second most smoked plant after tobacco. Wait a couple weeks for myclium to grow inside the liquid, put into a syringe with needle and inject into your substrate (or you could just pour it in). Then you can put spores or a small piece of mushroom into the liquid. You could make a liquid culture by mixing 1,500 ml of water, 1 tbsp raw honey and 1 tsp malt (then sterilize with a pressure cooker). However, you can use spores to create new strains of mushrooms and can be preferred to cultures sometimes because spore prints have been known to last for up to 17 years. When most people think of growing mushrooms from spores they think about psilocybe cubensis because it is legal to get those spores in most places for the purpose of studying them under a microscope and then people actually grow them (it is a federal crime in the United states to grow them). Essentially creating an endless supply of mushroom.īe sure to keep track of how many transfer you do though because the further you get away from the initial source the weaker the mycelium will get and eventually stop fruiting. Then when you start to run low on a plate you can transfer a small amount to another agar plate. Then, once you have your agar plates you can store them for 3 months to 3 years (depending on the species of mushroom). The advantage to this would be if there is any contamination it will be easily seen in the agar plate instead of wasting your bulk substrate. Option 2Īnother option would be to scrape some spores into an agar plate or liquid agar jar. Use promo code ‘ Curative ‘ for a 10% discount at checkout. Use promo code ‘ CURATIVEMUSH ‘ for a 20% discount at checkout.Ģ) The Mycelium Emporium (they sell liquid cultures in syringes). If you want to order edible or medicinal mushrooms you will find it much more common to order the liquid cultures that are already in a syringe or cultures on agar plates than to order spore prints…ġ) North Spore’s culture bank (they sell agar plate cultures). They should last about 4 to 5 months in the refrigerator (sometimes up to 12 months). You can order a pre-made spore syringe online. Storing spores in the refrigerator have been know to last for 18 years. Then you take a small portion of your spore print, mix it with some distilled water (same as sterile water) and suck into a syringe to make a spore syringe to use to inject into your substrate with a needle for the syringe. You can get your own spores by cutting the cap off a mushroom, putting it gill side down on a piece of foil and covering it with a glass for 12-24 hours. Then you can transfer the mycelium multiple times after that to other plates. The advantage of using an agar plate or agar liquid is to easily see if there is any contamination. Most of the time people will use rye grain, popcorn grain or saw dust in a small bag and wait for that bag to completely fill with mushroom mycelium which is called mushroom spawn. Some people do like to experiment with creating new strains so they prefer spores. Unless you want to create you own strain most people prefer to use mycelium since it is further along in the growing process and more predictable. How to use a spore print to grow mushrooms? If you use spores then you will be creating your own strain of that type of mushroom however if you are using mycelium you are essentially cloning a mushroom that already exists. Now when you want to grow mushrooms you can either use spores or mycelium to start it off. If you grow mushrooms from spore print you will need 2 spores to come together (almost like a sperm and egg) to start to grow the mycelium (mushrooms are made from mycelium). You might think it would be as easy as just getting the spores… While that is one option to do that, there are other options as well. Let’s take a look at the difference in more detail now…Ĭhoosing your mushroom source is kinda like deciding where to get the ‘seed’ (if you will) to grow it… Spawn is just a lot more of it than in culture. Whether you want to grow mushrooms from spore print, culture or spawn you will find that the process is much the same with each having its own separate advantage.Ĭultures and spawn are very similar in a sense that they are both mycelium. Most are more or less connected to the Gongyang Commentary and its school and written by a number of different persons at different times throughout the Han Dynasty. It seems safest to regard it as a collection of unrelated or loosely related chapters and shorter works, which could be subdivided into five categories. Scholars now reject as later additions all the passages that discuss five elements theory, and much of the rest of the work is questionable as well. Whether the work was written by Dong himself has been called into question by several scholars including Zhu Xi, Cheng Yanzuo, Dai Junren, Keimatsu Mitsuo, and Tanaka Masami. The Luxuriant Dew of the Spring and Autumn Annals bears many marks of multiple authorship. Another of his major works that has survived to the present is the Luxuriant Dew of the Spring and Autumn Annals in 82 chapters. There are two works that are attributed to Dong Zhongshu, one of which is the Ju Xianliang Duice in three chapters, preserved under the Book of Han. Temple in honor of Dong Zhongshu in Yangzhou He is also considered the originator of the doctrine of Interactions Between Heaven and Mankind, which lays down rules for deciding the legitimacy of a monarch as well as providing a set of checks and balances for a reigning monarch. He emphasised the importance of the Spring and Autumn Annals as a source for both political and metaphysical ideas, following the tradition of the Gongyang Commentary in seeking hidden meanings from its text. He appears to have been protected by the Emperor's chief counselor, Gongsun Hong.ĭong Zhongshu's thought integrated Yin Yang cosmology into a Confucian ethical framework. At one point he was thrown into prison and nearly executed for writings that were considered seditious, and may have cosmologically predicted the overthrow of the Han Dynasty and its replacement by a Confucian sage, the first appearance of a theme that would later sweep Wang Mang to the imperial throne. His relationship with the emperor was uneasy though. He entered the imperial service during the reign of Emperor Jing of Han and rose to high office under Emperor Wu of Han. His birthplace is associated with Wencheng Township ( 溫城鄉, now located in Jing Country), so in the Luxuriant Dew of the Spring and Autumn Annals he is once mentioned as Lord Dong of Wencheng ( 溫城董君). Biography ĭong was born in modern Hengshui, Hebei in 179 BC. However, he apparently enjoyed great influence in the court in the last decades of his life leading up to that. Ultimately banished to the Chancellery of Weifang by his adversary Gongsun Hong, Gongsun effectively promoted Dong's partial retirement from political life, and his teachings were transmitted from there. He apparently favored heaven worship over the tradition of cults celebrating the five elements. He is traditionally associated with the promotion of Confucianism as the official ideology of the Chinese imperial state. Dong Zhongshuĭong Zhongshu ( Chinese: 董仲舒 Wade–Giles: Tung Chung-shu 179–104 BC) was a Chinese philosopher, politician, and writer of the Han dynasty. In this Chinese name, the family name is Dong. 10, 225–257 (1986)Īrgyris, J.H., Faust, G., Szimmat, J., et al.: Recent development in the finite element analysis of pressure container reactor vessel. 42, 431–439 (2005)ĭesai, C.S., Somasundaram, S., Frantziskonis, G.: A hierarchical approach for constitutive modelling of geologic materials. 39, 695–729 (2002)Īl-Ajmi, A.M., Zimmerman, R.W.: Relation between the Mogi and the Coulomb failure criteria. 63, 12–26 (2013)Ĭolmenares, L.B., Zoback, M.D.: A statistical evaluation of intact rock failure criteria constrained by polyaxial test data for five different rocks. Liolios, P., Exadaktylos, G.: Comparison of a hyperbolic failure criterion with established failure criteria for cohesive-frictional materials. 45, 210–222 (2008)īenz, T., Schwab, R.: A quantitative comparison of six rock failure criteria. 48, 1233–1245 (2011)īenz, T., Schwab, R., Kauther, R.A., et al.: A Hoek–Brown criterion with intrinsic material strength factorization. Jiang, H., Wang, X.W., Xie, Y.L.: New strength criteria for rocks under polyaxial compression. Zhang, Q., Zhu, H.H., Zhang, L.Y.: Modification of a generalized three-dimensional Hoek–Brown strength criterion. Zhang, L.Y., Zhu, H.H.: Three-dimensional Hoek–Brown strength criterion for rocks. Yu, M.H.: Twin Shear Theory and Its Application. Singh, M., Raj, A., Singh, B.: Modified Mohr–Coulomb criterion for non-linear triaxial and polyaxial strength of intact rocks. Lee, Y.K., Pietruszczak, S., Choi, B.H.: Failure criteria for rocks based on smooth approximations to Mohr–Coulomb and Hoek–Brown failure functions. Zhang, Q., Wang, S.L., Ge, X.R., et al.: A modified Mohr–Coulomb strength criterion considering rock mass intrinsic material strength factorization. Lu, D.C., Du, X.L.: Research on nonlinear strength and failure criterion of rock material. Hosein, R.: New empirical polyaxial criterion for rock strength. Sriapai, T., Walsri, C., Fuenkajorn, K.: True-triaxial compressive strength of Maha Sarakham salt. Balkema, Rotterdam, August 30–September 2 (1989)Ĭhang, C., Hairnson, B.: True triaxial strength and deformability of the German Continental Deep Drilling Program (KTB) deep hole amphibolite. Takahashi, M., Koide, H.: Effect of the intermediate principal stress on strength and deformation behavior of sedimentary rocks at the depth shallower than 2000 m. Michelis, P.: Polyaxial yielding of granular rock. You, M.Q.: True-triaxial strength criteria for rock. In: Proceedings of the 5th North American Rock Mechanics Symposium and 17th Tunnelling Association of Canada Conference, Toronto, Canada, July 7–10 (2002) Hoek, E., Carranza-Torres, C.T., Corkum, B.: Hoek–Brown failure criterion-2002 edition. Griffith, A.A.: The phenomena of rupture and flow in solids. Mohr, O.: Welche Umstände bedingen die Elastizitätsgrenze und den Bruch eines Materials? Zeit. The proposed new true-triaxial strength criteria can provide theoretical foundation for stability analysis and optimization of support design of rock engineering. Moreover, the critical hydrostatic pressure \(I_\mathrm\) have more reasonable meridian and deviatoric function form, respectively. This is in accordance with intrinsic rock strength characterization. Mohr–Coulomb (MC), Hoek–Brown (HB), and Exponent (EP) criteria, the difference between generalized compression and extension strength of EP criterion experience a firstly increase then decrease process, and tends to be zero when hydrostatic pressure is big enough. Among three conventional strength criteria, i.e. The former two effects can be described by the meridian curves, and the last one mainly depends on the Lode angle dependence function. A reasonable strength criterion should reflect the hydrostatic pressure effect, minimum principal stress effect, and intermediate principal stress effect. Find top employees, contact details and business statistics at RocketReach.Item #: 1703-2. Style C Bracket - 3/16” Formed Wall Mount Bracketsįor flat walls or attach to pipe and round tube posts- with Style H adapter.R&B Wagner, Inc is a Wholesale Building Materials, Information Technology, and Handrail and Railing Systems company located in Milwaukee, Wisconsin with 187 employees. This Style B formed handrail bracket offers a 1 1/2” horizontal and vertical clearance to meet code requirements and the recommendations of the 2010 ADA Standards for Accessible Design (ADASAD). Redesigned to adjust the vertical clearance from the underside of the handrail to the horizontal arm of the bracket. Redesigned Style B Bracket with One Mounting Hole Note: Style B fillers can also be used for these style (page 5). Universal saddle for use with flat or round sections. Style B Bracket - 3/16” Formed Wall Mount Brackets Round Saddle, 3” Centerline, One, Two or Three Hole Universal Saddle, 3 1/4” Centerline, One, Two or Three Hole STANDARD PRODUCTS RAILING SYSTEMS LONG LENGTHS CUSTOM SERVICES Threaded Holes for -20 Mounting Screws 1 / 4 Also used with Style B brackets (page 6). Style B Filler Use filler to prevent overtightening of matching handrail bracket which may result in cracking the drywall. Two and three hole brackets must be countersunk to use these covers. Snap-On Cover For concealed mounting of single hole formed brackets. Bright polished, anodized, plated and galvanized finishes are available in stock or by request. Stainless steel brackets are supplied with a satin finish.Steel and aluminum brackets are unfinished.Snap-on covers are also available to cover the mounting hardware. Brackets available with one, two or three hole mounting options. People | Integrity | Empathy | Innovation | Performanceġ/4” Formed Wall Mount Brackets Available in steel, aluminum and stainless steel. Please confirm your local requirements, product dimensions and structural data prior to specifying. Note: not all products will meet all code requirements. Please visit for available pricing, inventory, dimensioned drawings and other product information. Wagner is proud to be your leading supplier of high-quality railing solutions and components. By standing firmly behind our products and services, we’ve earned the trust of our customers and continue to provide innovative solutions worldwide. We work diligently to ensure a quality customer experience for all Wagner partners and are passionate about finding you an efficient, high-quality solution that meets the needs and code requirements of your project. More than just a manufacturer and supplier, our niche focus and collaborative approach enables us to engineer high-quality, innovative systems and components based on customers’ needs. As an employee-owned company, we take pride in our employees for their hard work and commitment to quality. Why Choose Wagner? Wagner has been an authority and industry leader in metal fabrication and architectural railing since our founding in 1955. Most of our top sellers are in stock* and can ship today! Our Performance In stock catalog items are shipped same day if ordered before 1:00 p.m. Our Products We produce over 5,500 catalog parts with various customization options to ensure the perfect solution for your project. Our People Wagner Shareholders are committed to the quality of our products and customer experience. 114 ENDURALL™ Electrical Junction and Device Boxes. 100 Non-Weld, Slip-On Fittings Diamond Aluminum. If losing that much XP would cause you to lose a level, you instead lose an amount that leaves you with just enough XP to keep your level. This enmity lasts until either you or the devil dies.įool: You lose 10,000 XP, discard this card, and draw from the deck again, counting both draws as one of your declared draws. The devil seeks your ruin and plagues your life, savoring your suffering before attempting to slay you. You can use the card's magic as soon as you draw the card or at any other time before you die.įlames: A powerful devil becomes your enemy. The Fates: Reality's fabric unravels and spins anew, allowing you to avoid or erase one event as if it never happened. Only a god or the magic of The Fates card can end this curse. You take a -2 penalty on saving throws while cursed in this way. You draw no more cards.Įuryale: The card's medusa-like visage curses you. You can't be located by any divination magic, but a wish spell can reveal the location of your prison. You remain imprisoned until you are found and removed from the sphere. Everything you were wearing and carrying stays behind in the space you occupied when you disappeared. Otherwise, this card has no effect.ĭonjon: You disappear and become entombed in a state of suspended animation in an extradimensional sphere. If you are true neutral or unaligned, this card has no effect on you.Ĭomet: If you single-handedly defeat the next hostile monster or group of monsters you encounter, you gain experience points enough to gain one level. Lawful becomes chaotic, good becomes evil, and vice versa. * Found only in a deck with twenty-two cardsīalance: Your mind suffers a wrenching alteration, causing your alignment to change. Unless the card is the Fool or the Jester, the card reappears in the deck, making it possible to draw the same card twice. Once a card is drawn, it fades from existence. If you fail to draw the chosen number, the remaining number of cards fly from the deck on their own and take effect all at once. You must draw each card no more than 1 hour after the previous draw. Otherwise, as soon as you draw a card from the deck, its magic takes effect. Any cards drawn in excess of this number have no effect. Most (75 percent) of these decks have only thirteen cards, but the rest have twenty-two.īefore you draw a card, you must declare how many cards you intend to draw and then draw them randomly (you can use an altered deck of playing cards to simulate the deck). Usually found in a box or pouch, this deck contains a number of cards made of ivory or vellum. The principal is the amount you borrowed, while the interest is the sum you pay the lender for borrowing it. The major part of your mortgage payment is the principal and the interest. Typical costs included in a mortgage payment You can edit these amounts or even ignore them as you're shopping for a loan - those costs might be rolled into your escrow payment, but they don't affect your principal and interest as you explore your options. Bankrate's calculator also estimates property taxes, homeowners insurance and homeowners association fees. Your rate will vary depending on whether you’re buying or refinancing.Īs you enter these figures, a new amount for principal and interest will appear to the right. Our calculator defaults to the current average rate, but you can adjust the percentage. Next, you'll see “Length of loan.” Choose the term - usually 30 years, but maybe 20, 15 or 10 - and our calculator adjusts the repayment schedule.įinally, in the "Interest rate" box, enter the rate you expect to pay. You can enter either a dollar amount or the percentage of the purchase price you're putting down. A down payment is the cash you pay upfront for a home, and home equity is the value of the home, minus what you owe. In the "Down payment" section, type in the amount of your down payment (if you're buying) or the amount of equity you have (if you're refinancing). The calculus behind mortgage payments is complicated, but Bankrate's Mortgage Calculator makes this math problem quick and easy.įirst, next to the space labeled "Home price," enter the price (if you're buying) or the current value of your home (if you're refinancing). That way I can have my kids use dry-erase pens on them and use them again and again. I love a good simple activity, and word searches are at the top of my easy-peasy activity list! All you need to do is use my download link to download the printable, print it off at home or at your local print shop, andįor a little extra use, I often use my self-adhesive laminating sheets to quickly laminate the word searches. It’s easy to see already how these word searches are helping my kids:īringing out this first grade word search printable, or any of my other word searches always feels like a mom-win! And not to mention, they give me a free moment while my kids do something independently! How to Use This Word Search Printable So this first grade word search has been perfect! I can’t get enough of word searches for myself, but recently I’ve been loving them as activities for my kids too. Why You Need This First Grade Word Search And it’s such a fun activity too! Pull it out for a back-to-school activity, after-school snack game, or family game night. But I have such a hard time finding word searches that are child-oriented and simple enough for my kids to truly enjoy.īut this first grade word search printable is the perfect level for elementary school kids that are learning to read. Word searches are a great way to build some important skills for young kids. If you are a mom with a first-grader or you’re a teacher with a class full of grade one learners, then you definitely are going to want to grab my free first grade word search printable! If you know a first-grader you need to download this FREE First Grade Word Search Printable! It’s the most fun and simple way to boost word recognition and memory. You'll dial in anything you want on that. You can get on amazon, not sure where to test one out. I'll gladly champion the VHT special 6 over it (with a tube change once you get it probably 25 bucks and very simple). Your delay is just going to come from a pedal regardless. Many of you (I think) have blues juniors. Wondering about others in the price range for low wattage and bluesy. I wanna say most guitar stores are going to have those two in stock (or some sort of AC10) so try both out. Ok, ordered a Vox AC10C1 and got it today. Not sure any of the amps listed are country per se. You get your crunch on with it too for sure. There's a lot of control of the knobs to go from (Vox) cleans to dirty (Vox dirt that is). It's a nice alternative to the Fender amps I have. I don't know how it would sound for country though. It's better than the 7 for just a few buck more. It's got a master on it and reverb and a gain switch. The Cub-Super12 is a single-channel amp with two inputs. Again, its ceramic magnet is medium-weight everything about the Cub-Super12 is geared up to be portable. It has a HH Electronics H12-65 custom speaker. You can't go wrong.Bassbreaker 15 if you go with a bassbreaker, even if you have to wait to save for it. The Laney Cub-Super12 has a trio of 12AX7s in the preamp and pair of EL84 power tubes. If you can justify the cost, and are forced to get just one, go for the Princeton. It is a pleasantly surprising amp with complex tones that still puts a smile on my face every time I play it. If money is a concern, get the Vox and never look back. At home it still has that great Vox tone at low volume, and will give you the crunch you need, but is a little more selective as to which pedals work best. It's Blues tone is definitely more '60's British, but still lush. Its bright, loud, punchy, and can rock with the best of them. For live playing, I probably like it a bit more than the Princeton. But, remember, its twice the price of the Vox. It is a great example of a 6V6 power tube amp. You can shape more different tones with it, especially in a home, studio, or recording setting. It takes a wider range of pedals a little better than the Vox. The Princeton has more tonal range, meaning from a true Fender-y clean, to a wonderful, creamy distortion, to tremolo drenched surf style, to a glorious muted jazz tone. So, how to choose? If I was forced to choose, which fortunately I am not, it would be tough. Both have strong, defined low bass tone coming from the 10" speakers. The Fender has those beautiful cleans, and the Vox just chimes. But.for about the same amount of as the supersonic and with about the same power you could have a Deluxe Reverb. Especially the model with the Eminence Cannabis Rex speaker, which is a FSR from Sweetwater. IVs a good review some time back and, if I recall, said the IVs are superior to the earlier versions. I have a blues Jr which is a great little amp. Member TSims1 (whos opinion we all value) gave the new Blues Jr. Being lower wattage, they both are able to maintain their true character played down low in a home setting. I really dont like the supersonic - its an amp that looks like a fender but doesnt act like one IMHO. I love that either are "grab-and-go" for live playing. If the band is a really loud one, just put a PA microphone on either amp and you're good to go. They both hang in great in a small band scenario, and I have used both, un-miced, with two other guitarists and a drummer with no problem. If you plan on using pedals to get your drive, I’d suggest the Fender. If you plan on using the amp’s drive, I’d go Vox. First of all, both meet your criteria above. I like the Blues Junior cleans better but the Vox drive better. "Let me try to give you a more detailed impression of these two amps the Fender '68 Custom Princeton and the new VOX AC10C1. Its my best answer on these two great smaller amps: Most blues guitarists are looking for slightly overdriven tones, most of the time. As a result, you can push a small guitar amp into that beautiful bluesy breakup at a lower volume. Putting the Vox AC15c1 against the Fender Tweed Blues Jr (III). Here's what I wrote to a fellow member in December. Lower watt amps are less powerful, so you can overload the tubes within them more easily. |
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